Technical Director, Animal Transgenesis Core (Biomedical Sciences)

University Overview

The University of Pennsylvania, the largest private employer in Philadelphia, is a world-renowned leader in education, research, and innovation. This historic, Ivy League school consistently ranks among the top 10 universities in the annual U.S. News & World Report survey. Penn has 12 highly-regarded schools that provide opportunities for undergraduate, graduate and continuing education, all influenced by Penn’s distinctive interdisciplinary approach to scholarship and learning. As an employer Penn has been ranked nationally on many occasions with the most recent award from Forbes who named Penn one of America’s Best Large Employers in 2023.

Penn offers a unique working environment within the city of Philadelphia. The University is situated on a beautiful urban campus, with easy access to a range of educational, cultural, and recreational activities. With its historical significance and landmarks, lively cultural offerings, and wide variety of atmospheres, Philadelphia is the perfect place to call home for work and play.

The University offers a competitive benefits package that includes excellent healthcare and tuition benefits for employees and their families, generous retirement benefits, a wide variety of professional development opportunities, supportive work and family benefits, a wealth of health and wellness programs and resources, and much more.

Posted Job Title

Technical Director, Animal Transgenesis Core (Biomedical Sciences)

Job Profile Title

Associate Director E, Research

Job Description Summary

A technical director position is available at the Center for Animal Transgenesis in the Department of Biomedical Sciences at University of Pennsylvania School of Veterinary Medicine. This core provides genome editing services as well as an array of pre-implantation manipulation services, primarily in mice, to investigators both within and outside of the University of Pennsylvania (Penn).
The responsibilities of this position include but are not limited to: consultation with investigators, performing procedures, management of the core staff, compliance with regulatory procedures, IACUC protocols, and management of the core budget. The technical director is expected to directly carry out genome editing and mouse embryo manipulation procedures (and potentially manipulation of other species as well). Candidates should have a minimum of a Bachelor of Science degree in biology and extensive experience (10 yrs) and strong technical skills in mouse husbandry and genetic manipulation, including microinjection of mouse zygotes, mouse blastocyst injection, pronuclear injection, ICSI (intracytoplasmic sperm injection), somatic nuclear transfer, IVF, embryo cryopreservation, embryo transfer, and kidney capsule grafting. In addition, candidates should have a strong understanding of the knowledge basis of genome eidting. Highly motivated and diligent individuals with strong interpersonal skills are encouraged to apply to this position by providing a cover letter, CV, and names and contact information for three references.
University of Pennsylvania is located in the vibrant Philadelphia metropolitan district and is one of the top research universities in the world.

Highly skilled person proficient in the microinjection of mouse embryos who will work independently to perform all aspects of producing transgenic mice and supervise other lab personnel. Major responsibilities include generation of gene targeted and transgenic mice with skills to include microinjection of zygotes with CRISPR-Cas9 reagents, ES cell blastocyst injection, ES cell morulae aggregation, pronuclear injection of DNA, microsurgery, sperm and embryo cryopreservation, IVF, ICSI (mouse and horse), somatic cell nuclear transfer, germ cell transplantation, kindey capsule grafting, hormone injections for superovulation, checking plugs, and culturing primary and embryonic stem cells. Other requirements include the ability to troubleshoot research and optimize procedures, track relevant scientific literature, maintain detailed records/files.

Job Description

Job Responsibilities

• Microinjection of mouse zygotes (CRISPR-Cas9), mouse blastocyst injection, pronuclear injection, ICSI (intracytoplasmic sperm injection), somatic nuclear transfer, IVF, embryo cryopreservation, embryo transfer, and ES cell culture
• General mouse surgical and husbandry procedures (superovulation, kidney capsule grafting, etc)
• Consultation with investigators, management of the core staff, compliance with regulatory procedures and inspections, IACUC protocol renewal and amendment, and management of the core budget
• Other duties and responsibilities as assigned

Qualifications

• Bachelor of Science, Bachelor of Arts, and 7