Staff Research Associate

The Quantitative Biosciences Institute (QBI) Krogan Lab at UCSF is seeking a qualified and motivated Staff Research Associate II (SRA II) to join a highly collaborative and interdisciplinary research environment focused on neurodevelopmental disorders and protein interaction biology. This position is an excellent opportunity to learn several valuable, cutting edge techniques in molecular biology,neuroscience and proteomics, while working with an highly dynamic and interdisciplinary team. 

Under the general supervision of the research leadership, the SRA II will contribute to research projects focused on mapping protein interactions and pathways in different cell types, including iPSC-derived neurons. Experimental responsibilities include expression of affinity-tagged proteins, affinity purification and proximity labeling workflows for mass spectrometry-based protein interaction studies, mammalian cell culture maintenance, molecular cloning, and validation assays using cellular, molecular, and biochemical techniques. The position requires strong organizational skills, attention to detail, scientific curiosity, and the ability to work independently in a dynamic interdisciplinary team environment.

 

DUTIES & ESSENTIAL JOB FUNCTIONS

Identify the functions or tasks that employees in the job perform. The essential functions should state the purpose of the work and the results to be accomplished, rather than how the function is performed. Of the tasks listed, what percentage of time is devoted to each? The more time employees spend on a function, the more likely it is that the function is essential. Generally, include those functions that account for 10% or more of the work, i.e., key items that contribute significantly to the achievement of the job.  The functions should add up to 100%.

% 

of time

Essential Function (Yes/No)

  

Key Responsibilities

(To be completed by Supervisor)

45

YesPerform mammalian cell culture including maintenance of iPSC-derived neuronal cultures, assist with neuronal differentiation protocols and maintenance of relevant cell cultures..

45

YesConduct molecular biology and biochemical experiments and analysis including but not limited to molecular cloning, plasmid preparation, PCR, western blotting, expression of tagged proteins, immunoprecipitation, affinity purification, and proximity labeling workflows

10

YesOrdering and maintaining project-specific inventory

100%

 (To update total %, enter the amount of time in whole numbers (without the % symbol - e.g., 15, 20) then highlight the total sum (e.g., 1%) at the bottom of the column and press F9. The total sum should add up to 100%.)

 

  

 

  

 

  

Required Qualifications:

• Bachelor's degree in Biology, Biochemistry, Molecular Biology, Cell Biology, Neuroscience, Chemical Biology, or a related scientific discipline; and one or more years of recent, directly related work experience, utilizing the techniques or methods required by the position; and/or equivalent experience/training.

• Experience in mammalian cell culture techniques, including maintenance of iPSCs or related cell lines

• Experience with molecular biology techniques, including molecular cloning, plasmid preparation, PCR, western blotting, and sterile technique

• Ability to work independently, prioritize responsibilities, and meet deadlines in a fast-paced research environment

• Practical knowledge of laboratory operations, stock preparation, inventory management, and laboratory organization

• Ability to maintain and troubleshoot routine laboratory equipment

• Excellent organizational, verbal, and written communication skills

• Strong attention to detail, problem-solving skills, and ability to work collaboratively within interdisciplinary research teams

• Working knowledge of Microsoft Office applications

Preferred Qualifications:

• Experience with CRISPR gene editing techniques
• Experience with different gene delivery systems