Sr. Research Specialist

The Pathology Department, Neuropathology division is seeking a proficient Sr. Research Specialist with a sophisticated understanding of techniques and a solid foundation in the underlying science, gained from experience. Under minimal or no supervision, performs research laboratory experiments by developing new or following established procedures and protocols. Participates in the design of experiments and analyzes data. Works independently and manages daily research projects. Provides training to other individuals who may be assigned to the laboratory on a regular or temporary basis. The senior research specialist will assist the principal investigator in the development and coordination of specific research projects, experimentation, data collection and analysis, and organization of the laboratory.

Specific Duties & Responsibilities

Primary Research

• Performs experiments related to primary neuronal cultures, neuronal differentiation of human stem cells, genetic manipulation of the above, phenotypic characterization of the above with the indicated molecular and biochemical methodologies.
• Performs western blot, PCR, cloning experiments, and related biochemical and molecular essays.
• Participates in preparation of tissues and immunohistochemical and in situ hybridization probing of gene expression in tissues.
• Working with the PI, develops new methods or imports new methods from other entities inside and outside Hopkins, including academia and corporations, as deemed appropriate for research programs in the Koliatsos Lab.
• Assists the PI in grant preparation as needed.

Laboratory management & research coordination

• Assists the principal investigator to assign responsibilities and coordinate different projects and manages the laboratory operations. This is the go-to in the absence of the PI.
• Maintains database and organizes specimens and reagent resources.
• Is responsible for routine operational activities such as: ordering supplies and materials, reviewing inventory, maintaining up to date records of experiments/projects, etc.
• Monitors inventory levels and proper storage of materials and supplies in accordance with established policies and procedures.

Equipment, Machine, or Tool Requirements

• Computer and Laboratory equipment maintaining and operating including: cell culture systems, microtomes/vibratomes, centrifuges, electrophoresis devices, HPLC, mass spectrometers, spectrophotometers, freezers, etc.

Special Knowledge, Skills, & Abilities

• Expert in cell culture and molecular methods.
• Advanced knowledge of good laboratory practices.
• Great organizational skills.
• Excellent record keeping and documentation skills.
• Able to work independently, under minimal supervision with strong decision-making skills and good judgment.

Specific Physical Requirements

• While performing the duties of this job, the employee may remain in a stationary position (sitting or standing) up to 70% of the time.
• Occasionally required to traverse throughout the laboratory or to retrieve equipment, supplies, or specimens from other locations on campus.
• The employee is required to operate computer keyboard, laboratory instruments, and small laboratory tools such as pipettes, cutting instruments, glass tubes, dishes, and/or slides. Constantly repositions self to perform duties at laboratory work stations, gathering specimens, and loading equipment.
• Prolonged vision requirements including viewing computer screen, paper reports/documents, charts and results.
• Visual acuity is required to distinguish fine gradation of color or structure and closely examine specimens (including via a microscope).

Laboratory environment

• Exposure to toxins and infectious agents exist, but potential for personal injury or harm is minimized if established safety and health precautions are followed.
• Must refer to and are expected to comply with procedure manuals, follow proper laboratory protocols and safety policy/procedures, and be familiar with material data safety sheets in assigned work areas.
• Able to read and understand all Health, Safety and Environment (HSE) guidelines applicable to assigned work area.
• Working in laboratory where there may be discomforts due to odors, noise, temperature fluctuations, and working around lab equipment.
• Research areas may include animal, human, human products, DNA, radiation protocols, and other bio-hazardous materials.
• Use of personal protective equipment may be advised or required.
• May transport equipment and supplies usually less than 40 pounds from one area to another (in the laborato