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JOB DESCRIPTION AND POSITION REQUIREMENTS:

The Penn State College of Medicine, Heart and Vascular Institute, in Hershey, PA is immediately seeking a post-doctoral scholar to study the impact of arrhythmia and heart failure in heart disease. The research position requires a PhD or MD degree at the time of appointment. Our laboratory uses multidisciplinary approaches to study the structural and electrical components of cardiovascular function. Our research spans from human to cell models, to small animals. The ideal candidate will have significant experience in cell culture including overexpression, siRNA transfection, and preferably in working with human cardiomyocyte lines. In addition, experience in handling small animals, electrocardiography, echocardiography and surgical skills to implant radiotelemetry would be an asset. Applicants should have expertise in basic laboratory techniques to perform immunoblotting assays, fluorescence imaging, fluorescence-based studies employing a robotics microplate reader, quantitative PCR, gel electrophoresis and cell culture techniques. The successful candidate must demonstrate technical and theoretical knowledge of the aforementioned assays. As a consequence, the candidate will be expected to analyze the data collected and present the results in formal presentations, manuscripts and grant applications.

Primary Duties

% of time

Position Summary

40-50%

Cell culture of commercially available and iPSC derived human cells lines, specifically cardiomyocytes. Prepare clones for transfection (overexpression) as well as executing siRNA knockdown lines and maintaining cultures. Prepare cardiomyocytes and perform contraction analyses at rest and in response to medication studies. Peform membrane potential and ion transient analysis in WT resting cells and with agonist administration, including dose response studies.

10-20%

Prepare cDNA clones for transfection, prepare dissection and dissociation solutions, process DNA and protein samples for PCR and Western blotting assays. Maintain cell line cultures for transfection. Transfect cell lines, perform pharmacological assays of transfected cell lines with the microplate reader, perform Western blotting assays and quantitative RT-PCR assays.

10%

Perform small animal cardiac structure and functional analysis. This includes independently performing small animal echocardiography, real-time measurements of function using 2D, M-mode, & Doppler technology, as well as analysis of data. Perform radiotelemeter implantation, resting electrocardiographic s