Senior Scientist – Ex Vivo Patient-Derived Platforms & FACS

About AstraZeneca 

AstraZeneca is a global, science-led biopharmaceutical company that focuses on the discovery, development, and commercialization of prescription medicines in Oncology, Rare Diseases, and BioPharmaceuticals. Our mission is to push the boundaries of science to deliver life-changing medicines. 

Position Overview 

We are seeking an exceptional Senior Scientist to join our Oncology Translational Science team. This role will focus on advancing our understanding of tumor immunology and therapeutic response through sophisticated ex vivo patient-derived platforms and multi-parameter flow cytometry. The ideal candidate will drive innovation in assay development, lead translational research initiatives for Tier 1 assets, and contribute to predictive biomarker strategies that bridge preclinical findings with clinical outcomes. 

Key Responsibilities 

Ex Vivo Platform Development & Execution 

• Lead end-to-end workflows for patient-derived tumor platforms including PDTF (patient-derived tumor fragments) and tissue slice cultures 

• Design and execute ex vivo pharmacodynamic profiling studies to evaluate AstraZeneca and competitor therapeutic candidates 

• Establish robust tumor/immune cell co-culture systems to assess therapeutic efficacy, including cytotoxicity, immune cell activation, and target engagement 

• Process and analyze primary patient specimens with meticulous attention to sample integrity and experimental reproducibility 

Advanced Flow Cytometry & Immunophenotyping 

• Design, optimize, and implement high-dimensional spectral flow cytometry panels (20-30+ colors) for comprehensive immune profiling of patient tumors 

• Conduct antigen density quantification, intracellular signaling assessments, and functional immune cell characterization 

• Perform single-cell sorting, fluorescent antibody labeling, and specialized applications including trogocytosis and proliferation assays 

• Utilize platforms including Cytek Aurora, BD FACSymphony, and other state-of-the-art cytometry instruments 

Assay Development & Optimization 

• Develop and validate novel flow cytometry-based assays to evaluate immune cell function, T-cell modulation, and therapeutic mechanisms of action 

• Optimize cellular assays including cytotoxicity, phosphorylation, proliferation, and cytokine secretion readouts 

• Generate stable cell lines using lentiviral transduction and electroporation-based methods for target validation and assay development 

• Establish CAR-T cell generation protocols and functional evaluation pipelines 

Translational Research & Data Integration 

• Integrate multi-modal readouts (flow cytometry, cytokine profiling, target engagement) to provide comprehensive pharmacodynamic assessments 

• Develop predictive models linking ex vivo responses to clinical outcomes, supporting patient stratification strategies 

• Collaborate with computational biology t