Core Senior Research Specialist

This position serves as a senior technical expert to provide core/lab management support of Salk Institute Mass Spectrometry Core services. The incumbent, with limited input from the Core Director, will independently conduct experiments related to LC/MS technologies for proteomics. Responsibilities include independent development and execution of specific workflows for sample preparation including protein fractionation, sample cleanup (precipitation, SPE, and affinity column), in-gel and in-solution protein digestion, lyophilization, stable isotope peptide labeling (i.e. TMT), and enrichment of post-translationally modified peptides.

Responsibilities also include preparation of capillary LC columns, operation of instrument software for data acquisition by LC/MS and usage of bioinformatics software for protein identification and quantification. The position will support the Core Director with management of core operations, including its resources, executing administrative duties, supporting grant writing and training programs, and maintaining lab protocols. Secondarily, the position will engage in sample preparation and analysis for metabolomics and lipidomics applications.  

Who We Are

The Salk Institute is an internationally renowned research institution that values diversity, equity, and inclusion. We seek bold and interactive leaders passionate about exploring new frontiers in science. Our collaborative community embraces diverse perspectives and unique life experiences, fostering innovation, and a sense of belonging. Together, we strive to improve the wellbeing of humanity through groundbreaking research.

Founded by Jonas Salk, developer of the first safe and effective polio vaccine, the Institute is an independent, nonprofit research organization and architectural landmark: small by choice, intimate by nature, and fearless in the face of any challenge. Salk's vibrant community comprises a diverse and talented group of individuals, each playing a crucial role in driving our mission forward. From visionary leaders to dedicated administrators and brilliant faculty members, the Institute is united by a shared passion for scientific exploration and innovation.

What Your Key Responsibilities Will Be

• Works with Salk researchers to achieve targeted research goals utilizing mass spectrometry (MS) technology platforms for proteomics analysis. Performs procedures associated with protein/peptide identification and quantitative analysis, including sample preparation, instrument operation and data analysis. Provides collaborative support to experiment design and implementation for new and existing approaches to using MS technologies for proteomics analysis. Collaborates and assists Salk researchers on application and development of advanced MS methods for proteomic analysis.   60%
• Supports the Core Director with sample preparation and analysis for metabolomics and lipidomics applications. 15%
• Maintains core facility to include mass spectrometry equipment and instrumentation maintenance and scheduling repairs, oversight of inventory and ordering, instrument QA/QC, data management and record keeping, and overseeing lab safety protocols and practices. 10%
• Reviews current MS-based technologies, particularity in the fields of proteomics by review of relevant literature and attendance at scientific meetings. Reviews the latest in technology (hardware, software and networking) and ensure that recommendations for changes or upgrades will integrate well with existing MASS core technologies. 5%
• Participates in teaching, training and instruction to a broad spectrum of researchers and core users. 5%
• Provides relevant support for grant applications, progress reports, and support for the development and design of training programs, workshops, and seminar series when appropriate. 5%

SUPERVISORY RESPONSIBILITIES:

May provide supervision, training and guidance to lower-level personnel.

What we Require

• Bachelor’s degree in relevant field with 7+ years’ experience, OR graduate degree (M.S. or Ph.D.) with 5+ years’ experience.
• 5+ years of experience in mass spectrometry, to include experience in the design, execution and analysis of mass spectrometry-based experiments for proteomic analysis on current technology platforms, especially Orbitrap systems.
• Experience and detailed understanding of a range of methodologies utilizing MS technology including protein an