Senior Molecular Biology Associate I *PC 1406

Your Role:

The role will support the development of new plasmid DNA purification processes for preclinical and GMP manufacturing. The role will also involve supporting ongoing construct assembly and plasmid production. This position requires a hands-on approach and relies on strong expertise in bacterial systems, plasmid purification, and molecular biology techniques to help optimize processes, design experiments, analyze data, and prepare technical documentation within a collaborative, cross-functional environment.

Essential Duties and Responsibilities: 

• Perform molecular biology assays, including qPCR, ddPCR, restriction enzyme digest, and other related analytical techniques.
• Execute on plasmid DNA production activity according to defined Standard Operating Procedures (SOPs) and Batch Production Records (BPRs).
• Develop, optimize, and troubleshoot plasmid purification processes with a focus on scalability and GMP readiness.
• Author and review protocols, reports, and standard operating procedures (SOPs). 
• Perform molecular cloning and construct assembly, including restriction digests, ligations, transformations, and clone screening.
• Design DNA constructs using molecular biology software and analyze sequencing data to confirm construct identity and integrity.
• Maintain accurate experimental records in accordance with cGMPs.
• Coordinate preventive and emergency maintenance, while demonstrating proper equipment use.
• Assist the Department in identifying and executing ongoing improvement initiatives to meet operational objectives.
• Collaborate effectively within the molecular biology team and across cross-functional teams.

Requirements:

• Bachelor’s degree in molecular biology, Biochemistry, Biotechnology, or a related scientific discipline with +5 years of hands-on experience. 

• Hands-on experience with plasmid DNA purification. 

• Experience with molecular biology techniques, including cloning, restriction enzyme digestion, sequencing analysis, qPCR, and ddPCR. 

• Experience designing and assembling constructs using molecular biology and sequence analysis software. 

• Experience supporting process development or optimization activities in a PD/R&D environment. 

• Familiarity with GMP concepts and regulated documentation practices. 

 Skills: 

• Strong working knowledge of bacterial culture protocols, including aseptic technique and safe handling of biological materials. 

• Strong attention to detail, organization, and data integrity.

• Clear written and verbal communication skills and ability to work in a team-based environment. 

Physical Demands:

The physical demands described here are representative of those that must be met by an employee to successfully perform the essential functions of this job. Reasonable accommodations may be made to enable individuals with disabilities to perform the essential functions. While performing the duties of the job, the employee may frequently stand, walk, reach, handle, grasp, feel and talk. The job may require the employee to use eye/hand/foot coordination. The employee may occasionally lift and/or move, carry, push, up to 50 lbs of weight. The employee may occasionally be required to balance, stoop, kneel, crouch, and crawl. Specific vision abilities required by this job include close vision, color vision, peripheral vision, depth perception and ability to adjust focus. Employee must have the ability to handle more than one task at a time and must work at a rapid pace while maintaining attention to detail.

Working Environment:

The work environment characteristics described here are representative of those an employee encounters while performing the essential functions of this job. This job is performed in a temperature controlled, handicap accessible facility. The noise level in the work environment is usually moderate. All persons performing this job will receive appropriate training prior to engaging in work requiring PPE.  Frequently, used research/clinical equipment may be handled in this job and strict SOPs and PPE must be worn. Occasionally, this position deals with temperature-controlled materials in which personal protective equipment (PP