Sr. Research Specialist

The Pathology Department is seeking a proficient Sr. Research Specialist with a sophisticated understanding of techniques and a solid foundation in the underlying science, gained from experience. Under minimal or no supervision, performs research laboratory experiments by developing new or following established procedures and protocols. Participates in the design of experiments and analyzes data. Works independently and manages daily research projects. Will present at departmental/divisional meetings, and provides training to other individuals who may be assigned to the laboratory on a regular or temporary basis. The senior research specialist will assist the principle investigator in the development and coordination of specific research projects, experimentation, data collection and analysis, and organization of the laboratory.

Specific Duties & Responsibilities

Primary Research

• Performs experiments related to primary cultures, differentiation of human stem cells, genetic manipulation of the above, phenotypic characterization of the above with the indicated molecular and biochemical methodologies.
• Performs western blot, PCR, cloning experiments, and related biochemical and molecular essays.
• Participates in preparation of tissues and immunohistochemical and in situ hybridization probing of gene expression in tissues.
• May assist in surgical procedures and pharmacological treatments of small laboratory animals.
• Working with the PI, develops new methods or imports new methods from other entities inside and outside Hopkins, including academia and corporation, as deemed appropriate for research programs in the James Lab.
• Performs the quality control evaluation and data analyses using computational tools.
• Creates and implements laboratory standard operating procedures (SOP) of all aspects of sample handling and analysis.
• Records outcomes/results of all experiments by writing.
• Communicates results in lab and institutional meetings and scientific conferences.

Laboratory Management and Research Coordination

• Maintains database and organizes specimens and reagent resources.
• Monitors biohazard/radiation safety standards and proper handling of potentially hazardous chemical and biological agents and/or radiation sources in the workplace.
• Does electronic billing entry for work done in lab.
• Is responsible for routine operational activities such as: ordering supplies and materials, reviewing inventory, maintaining up to date records of experiments/projects, etc.
• Monitors inventory levels and proper storage of materials and supplies in accordance with established policies and procedures.
• Performs maintenance of equipment.
• Performs miscellaneous related duties as assigned.

Educational/Communication Functions

• Exchanges information in an appropriate manner requiring good oral and written communication.
• Trains students, staff members, and postdoctoral fellows in sample preparation and instrument operation with established SOPs.
• Has regular interactions with students, post-doctoral fellows, faculty members, and vendors. Oversees day to day activity of Post Docs and students in the lab.
• Leads collaborative lab activities, representing the James Lab and Johns Hopkins in a professional manner.

Equipment, Machine, or Tool Requirements

• Computer and Laboratory equipment maintaining and operating including: cell culture systems, microtomes/vibratomes, centrifuges, electrophoresis devices, HPLC, mass spectrometers, spectrophotometers, freezers, etc.

Special Knowledge, Skills, and Abilities

• Advanced knowledge of good laboratory practices.
• Great organizational skills.
• Excellent record keeping and documentation skills.
• Able to work independently, under minimal supervision with strong decision making skills and good judgment.

Specific Physical Requirements

• While performing the duties of this job, the employee may remain in a stationary position (sitting or standing) up to 70% of the time.
• Occasionally required to traverse throughout the laboratory or to retrieve equipment, supplies, or specimens from other locations on campus.
• The employee is required to operate computer keyboard, laboratory instruments, and small laboratory tools such as pipettes, cutting instruments, glass tubes, dishes, and/or slides. Constantly repositions self to perform duties at laboratory work stations, gathering specimens, and loading equipment.
• Prolonged vision requirements including viewing computer screen, paper reports/documents, charts and results.
• Visual acuity is required to distinguish fine gradation of co