Staff Research Associate

The Quantitative Biosciences Institute (QBI) Krogan Lab at UCSF is seeking a qualified and motivated Staff Research Associate II (SRA II) to join a highly collaborative and interdisciplinary research environment focused on neurodevelopmental disorders and functional proteomics. 

Under general supervision of the research leadership, the incumbent will assist in mapping protein interactions and pathways in various cell types involved in neurodevelopmental disorders. This role is an excellent opportunity to learn several valuable, cutting edge techniques in molecular biology,neuroscience and proteomics. Research responsibilities include mammalian cell culture and maintenance; molecular cloning; affinity purification and proximity labeling experiments; expression of tagged proteins and patient-derived variants; preparation of samples for downstream mass spectrometry analysis; and application of molecular, cellular, and biochemical assays to validate experimental findings.

The ideal candidate is highly organized, detail-oriented, and table of balance multiple priorities in a fast-paced research environment. Strong problem-solving abilities, communication skills, and the ability to work independently and collaboratively within interdisciplinary teams are essential.

 

DUTIES & ESSENTIAL JOB FUNCTIONS

Identify the functions or tasks that employees in the job perform. The essential functions should state the purpose of the work and the results to be accomplished, rather than how the function is performed. Of the tasks listed, what percentage of time is devoted to each? The more time employees spend on a function, the more likely it is that the function is essential. Generally, include those functions that account for 10% or more of the work, i.e., key items that contribute significantly to the achievement of the job.  The functions should add up to 100%.

% 

of time

Essential Function (Yes/No)

  Key Responsibilities

(To be completed by Supervisor)

45YesPerform mammalian cell culture including maintenance of iPSC-derived neuronal cultures, assist with neuronal differentiation protocols and maintenance of relevant cell cultures..45YesConduct molecular biology and biochemical experiments and analysis including but not limited to molecular cloning, plasmid preparation, PCR, western blotting, expression of tagged proteins, immunoprecipitation, affinity purification, and proximity labeling workflows10YesOrdering and maintaining project-specific inventory100% (To update total %, enter the amount of time in whole numbers (without the % symbol - e.g., 15, 20) then highlight the total sum (e.g., 1%) at the bottom of the column and press F9. The total sum should add up to 100%.)         

252015201055100%

Required Qualifications:

• Bachelor's degree in Biology, Biochemistry, Molecular Biology, Cell Biology, Neuroscience, Chemical Biology, or a related scientific discipline; and one or more years of recent, directly related laboratory experience; or an equivalent combination of education and experience.
• Experience with mammalian cell culture techniques, including sterile technique and routine maintenance of cultured cell lines.
• Experience performing molecular biology techniques including molecular cloning, plasmid preparation, PCR, and protein analysis methods such as Western blotting.
• Experience preparing laboratory reagents, stock solutions, buffers, and media according to established protocols.
• Experience maintaining accurate laboratory records and following established experimental protocols.
• Knowledge of laboratory operations, including inventory management, ordering supplies, equipment coordination, and laboratory safety practices.
• Ability to operate and maintain standard laboratory equipment, including centrifuges, incubators, thermocyclers, plate readers, and related instrumentation.
• Demonstrated ability to work independently, prioritize multiple assignments, meet deadlines, and effectively troubleshoot routine laboratory issues.
• Strong written and verbal communication skills and the ability to work effectively in a collaborative, interdisciplinary research environment.

• Proficiency with Microsoft Office applications, including Word, Excel, and PowerPoint) 

Preferred Qualifications:

• Experience with CRISPR gene editing techniques
• Experience with different gene delivery systems