UNIV-Research Specialist II-Department of Biochemistry
Medical University of South CarolinaAbout the role
Job Description Summary
The department of Biochemistry & Molecular Biology at MUSC has an opening for a motivated and collegial Research Assistant in the laboratory of Dr. Jessica Hartman. The Hartman lab focuses on the impact of lifestyle factors such as diet and exercise on metabolism, with a special focus on the mammalian enzymecytochrome P450 2E1 (CYP2E1). The lab uses the model organism C. elegans (a microscopic roundworm), as well as mammalian cell culture and rodent models. We are looking for a candidate with excellent organizational skills, prior experience in a laboratory environment, and the ability to work well on a fast-paced and high-performing team. Previous experience in the relevant models is appreciated, but not required. Training will be provided in all aspects of the work.Entity
Medical University of South Carolina (MUSC - Univ)Worker Type
EmployeeWorker Sub-Type
Research GrantCost Center
CC000954 COM BIOCHEM Operations CCPay Rate Type
HourlyPay Grade
University-GEN08
Pay Range
Scheduled Weekly Hours
40Work Shift
Job Description
Job Description:
The department of Biochemistry & Molecular Biology at MUSC has an opening for a motivated and collegial Research Assistant in the laboratory of Dr. Jessica Hartman. The Hartman lab focuses on the impact of lifestyle factors such as diet and exercise on metabolism, with a special focus on the mammalian enzymecytochrome P450 2E1 (CYP2E1). The lab uses the model organism C. elegans (a microscopic roundworm), as well as mammalian cell culture and rodent models. We are looking for a candidate with excellent organizational skills, prior experience in a laboratory environment, and the ability to work well on a fast-paced and high-performing team. Previous experience in the relevant models is appreciated, but not required. Training will be provided in all aspects of the work.
Job Duties:
(20%) Performs mammalian tissue culture
- Grows, maintains, expands, and treats mammalian cell lines
- Develops, evaluates and implements new or modified culture procedures
- Improves laboratory methods of primary tissue culture
(20%) Performs DNA/RNA/Protein analysis
- Extracts DNA/RNA/Protein from mammalian cells/tissues.
- Analyzes DNA/RNA/Protein by PCR, Northern Blot, and Western Blot
(20%) Performs C. elegans culture
- Grows, maintains, expands, and treats worm lines
- Develops, evaluates and implements new or modified culture procedures
- Improves laboratory methods of worm culture
(15%) Prepares laboratory records
- Prepares daily records of experimental procedures and results
(10%) Assist DLAR with maintenance of mouse colony
- - Manage mouse colony numbers and supervise breeding schedules
- PCR genotyping of mice tail clippings
- Assist animal experiments for other lab members
(10%) Prepares laboratory records and maintain laboratory supplies
- Orders and places laboratory supplies
(5%) Upon request, performs other tasks requested by faculty to maintain a smooth operation of laboratory
- Performs collaborations with inside and outside colleagues
Minimum Requirements:
Required to have a minimum Bachelor’s degree in Biology or related subjects. Preferred to have Master’s degree or previous research experience in mouse breeding and colony maintenance.
Additional Job Description
Minimum Requirements: A bachelor's degree in chemistry, biology, or other natural, life or health care science and one year work experience directly related to the assigned research area. Physical Requirements: (Note: The following descriptions are applicable to this section: Continuous - 6-8 hours per shift; Frequent - 2-6 hours per shift; Infrequent - 0-2 hours per shift) Ability to perform job functions in an upright position. (Frequent) Ability to perform job functions in a seated position. (Continuous) Ability to perform job functions while walking/mobile. (Frequent) Ability to climb, using step stool. (Infrequent) Ability to work in temperature extremes (32° F to 100° F) when required due to assignments in cold rooms and/or foreign countries. (Infrequent) Ability to work in confined/cramped spaces. (Continuous) Ability to bend at the waist. (Frequent) Ability to twist at the waist. (Frequent) Ability to fully use both hands/arms. (Continuous) Ability to perform repetitive motions with hands/wrists/elbows and shoulders. (Frequent) Ability to fully use both legs. (Frequent) Ability to reach in all directions, stretch, bend, while moving items/materApply for this role
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