Laboratory Sciences Principal Professional

University of Colorado Anschutz Medical Campus

Department: Gates Institute

Job Title: Laboratory Sciences Principal Professional

Position #:00836518 – Requisition #:37176

Job Summary:

A Laboratory Sciences Principal Professional position is available to assist the Principal Investigators (PI) on multiple projects and oversee general laboratory operations and mouse colony at the Gates Institute. We are looking for a candidate to join our team who is proficient in the field of cellular reprogramming and stem cell differentiation and who is passionate about translational research, developing therapies and diagnostic tools for rare conditions. Our research focuses on a variety of projects related to regenerative medicine, cellular aging, and stem cell therapy development. Among the projects, the department develops an induced pluripotent stem cell-based models for studying Ehlers Danlos Syndrome and testing therapies for this condition and other conditions affecting the skin and connective tissues.

The team works closely with the Gates Biomanufacturing Facility (GBF) in advancing cell therapies developed by the laboratory into clinical manufacturing. In the laboratory, the department utilizes induced pluripotent stem cells (iPSCs), CRISPR/Cas9, mouse xenograft models, human cell culture techniques, organoids, and other cell and molecular biology techniques. The successful candidate will perform molecular and cellular biology experiments such as immunohistochemical staining, western blotting, molecular cloning, cell culture, qPCR, ddPCR, differentiation of pluripotent stem cells into a variety of cell types, and CRISPR-Cas-mediated gene targeting. Laboratory duties will involve receiving laboratory reagents and making stock solutions. The candidate will also oversee the mouse colony and perform animal survival surgeries. This position requires independent judgment in daily operations and in solving non-routine problems. Development of new techniques and independence will be expected and encouraged. The laboratory environment is team-oriented, where creativity, enthusiasm and dedication are rewarded. In addition, the candidate is expected to assist the Stem Cell Biobank and Disease Modeling Core in performing services related to cell reprogramming and gene editing as needed. Furthermore, as the Principal Professional, the candidate will serve as a subject matter expert (SME) in cellular reprogramming and iPSC differentiation, leveraging their expertise to educate and train Core users and collaborators. The candidate will act as a consultative resource, providing guidance on experimental design, optimization strategies, and data interpretation related to cellular reprogramming, iPSC differentiation, and associated methodologies.

Key Responsibilities:

• Order supplies for daily operations of the laboratory and core using CU Marketplace and purchasing system.
• Prepare lab for daily operations, including stocking materials, equipment, safety, cleanliness, and related areas.
• Manage procurement and cost analysis.
• Write animal, IRB and biosafety protocols.
• Work with a mouse colony and perform mouse survival surgeries
• Oversee independent research projects and plan the scope of work for timely project fulfillment.
• Support the analytical testing process using standard lab support knowledge in the collection and review of conditions, including preserving samples, completing sample preparation, documenting, a