UNIV - Staff Scientist II - Pharmacology and Immunology

Job Description Summary

The Mass Spectrometry Facility, part of the I3 Omics Umbrella Core at the Medical University of South Carolina (MUSC), is seeking an experienced and self-driven proteomics expert to join our highly collaborative and supportive team. This Senior Scientist (SSII) position will assist in develop of a multi-omic platform for basic and translational research projects for MUSC investigators and external researchers. This will include developing state-of-the-art proteomic capabilities and facilitating the establishment of multi-omic platform including proteomics and metabolomics. Substantial experience in LC-MS/MS (Orbitrap & Astral MS)-based proteomic data acquisition, data processing, statistical and bioinformatic evaluation is required. Development of customized and/or state-of-the-art biochemical methods for sample preparation (for post-translational modifications, plasma proteomics, TMT-based proteomics, nDIA) is essential. Evaluation and quality assessment of proteomic workflows to ensure high quality data is necessary. Excellent communication skills are required for working with investigators in the areas of cancer, cardiovascular disease, neurodegenerative disease, and digestive and liver disease. Instrumentation in the Mass Spectrometry Facility includes a Thermo Scientific Easy1200 nLC-Orbitrap Fusion Lumos ETD/UVPD MS, Easy1200 nLC Exploris 480 MS, Vanquish Neo LC-Orbitrap Astral Zoom MS, Vanquish HPLC, Kingfisher Apex.

Entity

Medical University of South Carolina (MUSC - Univ)

Worker Type

Employee

Worker Sub-Type​

Unclassified

Cost Center

CC001034 COM PHARM Operations CC

Pay Rate Type

Salary

Pay Grade

University-00

Pay Range

0.00 - 0.00 - 0.000

Scheduled Weekly Hours

40

Work Shift

Job Description

The Proteomics Center encompasses a multi-team environment with proteomics, glycomics, glycoproteomics, and metabolomics analyses using LC-MS/MS instrumentation at the Mass Spectrometry Facility and Mass Spectrometry Imaging instrumentation in the Proteomics Center.

Job Duties:

·         35% Collaborate with multiple labs to design and execute proteomics projects. Plan, develop and execute proteomic experiments to establish state-of-the-art workflows. 

·         35% Analyze and interpret proteomic MS data acquired by DDA, DIA, nDIA and TMT using state-of-the art bioinformatic workflows.

·         10% Prepare reports, co-author publications and present results at meetings and/or conferences. Contribute data to grant applications. 

·         10% Assist in maintaining and troubleshooting LC-MS instrumentation and perform rigorous routine instrument quality control steps.

·         10% Assist with establishment of metabolomic services

 

MUSC Minimum Training and Experience:

A Ph.D., M.D. or equivalent with two years postdoctorate experience plus evidence of scholarly activity as demonstrated by at least five first author publications.

Preferred Experience:

• At least 5 years of proteomics experience.

• Ph.D in biological sciences, chemistry, or analytical chemistry.

•   Experience and knowledge of the operation and maintenance of liquid chromatography and mass spectrometry systems, especially Thermo Orbitraps and Astral MS.

• Extensive knowledge and practical experience with complex sample preparation for mass spectrometry workflows.

• Extensive experience in quantitative mass spectrometry-based strategies for proteomic analyses. Hands-on experience with computational tools for proteomics data analysis including MSFragger, MaxQuant, ProteomeDiscoverer, and Spectronaut or DIA-NN.

Preferred Knowledge, Skills, and/or Abilities:

• Working knowledge of modern molecular biology and biochemistry techniques such as chromatography, transfection, Western blots, and immunoprecipitation.

• Basic understanding of R programming.

• Strong written and oral communication skills in English.

• Work positively and enthusiastically as part of a team.

 

 

Salary and Benefits: The current salary range is commensurate with experience and accom