Scheduled Hours

40

Position Summary

The Perlmutter lab is seeking a talented, highly motivated individual to join our team in the Division of Gastroenterology, Hepatology, & Nutrition of the Department of Pediatrics at Washington University School of Medicine. The lab’s focus is research on the pathobiology of, and treatments for, α1antitrypsin deficiency (ATD), a rare disease in which the misfolded α1antitrypsin protein causes liver fibrosis/cancer by gain-of-toxic function and chronic obstructive lung disease by loss-of-function. One of our major areas of focus is discovery and testing of autophagy enhancer drugs to clear the toxic misfolded protein from the diseased liver.

Over the last 10 years the lab has refined the use of cell line and mouse models for testing of new drug strategies, now focusing mainly on autophagy enhancer drugs. This work has been informed by a novel C. elegans model of antitrypsin deficiency that has been adapted to high-content screening of drug and RNAi libraries for new therapies for other protein aggregation disorders. The lab has now identified a lead candidate autophagy enhancer drug with in vivo efficacy for ATD and also efficacy in cellular models of Huntington’s disease and frontotemporal dementia (tauopathy), therein leading us to also believe this drug series will be ideal for the tauopathy of chronic traumatic encephalopathy.

Designs research protocols. Assists in developing and conducting research projects, including experiment design, data analysis and documentation of experiment results.

Job Description

Primary Duties & Responsibilities:

More information about the Perlmutter Lab and recent publications is available here: https://pediatricgi.wustl.edu/items/perlmutter-lab/ 
PMID: 38557779, 38336172, 38066314, 37214956, 36848094, 36303071, 35301011

• Following instructions and discussions with the principal investigator, designs research protocols, including developing procedures for the collection, verification and management of data.
• Assists with grant preparation and reporting.
• Performs complex statistical analysis of data collected and writes interpretative reports. Verifies the correctness of the data submitted and makes recommendations based on these analyses.
• Documents research topics and prepares and submits papers based on research work to publications and committees for publication or presentation to peers.
• Solves practical problems relating to difficulties with equipment or test subjects. Suggests technical or procedural improvements in testing methods.
• Conducts literature searches related to research project.
• Performs other duties as assigned.

Working Conditions:

• Works in a laboratory environment with potential exposure to biological, radioactive, and chemical hazards.

• Must be physically able to wear protective equipment and to provide standard care to research animals.

The above statements are intended to describe the general nature and level of work performed by people assigned to this classification. They are not intended to be construed as an exhaustive list of all job duties performed by the personnel so classified. Management reserves the right to revise or amend duties at any time.

Required Qualifications

Education:

Master’s degree or combination of education and/or experience may substitute for minimum education.

Certifications:

No specific certification is required for this position.

Work Experience:

Laboratory Operations (3 Years)

Skills:

Not Applicable

Driver's License:

A driver's license is not required for this position.

More About This Job

Preferred Qualifications

Prior significant experience with at least one (ideally both) of the following model systems and techniques:

• Mammalian cell culture analyses, including quantification of effects of gene knockdown and overexpression on transcriptomic (RT-qPCR, RNA-Seq) and proteomic (immunoblot, immunohistochemical) changes. 
• Mouse model analyses, including mouse husbandry, colony maintenance, database management, drug dosing, vector-based hepatic gene overexpression, dietary manipulation, euthanasia, biosample (i.e., tissue, serum) collection and processing, for transcriptomic (bulk and single cell), proteomic, and metabolic analyses.

Additional Beneficial Qu