Scientist/Sr. Scientist, Protein Engineering

About Us
Evercrisp Biosciences is a well-funded Bay Area biotechnology company (moving to South San Francisco in Jan 2024) focused on the discovery and development of protein-based genome editors as therapeutics for multiple disease areas. Evercrisp Biosciences is applying advanced computational methods, machine-learning, and protein engineering to deliver novel enzymes with high cell uptake and in vivo editing. Our Protein Sciences team is seeking a highly motivated and energetic scientist to drive protein engineering efforts and lead biophysical characterization of our therapeutic molecules. The successful candidate will have the opportunity to make fundamental contributions to our platform. Evercrisp Biosciences is a dynamic and well-funded research organization that offers a highly competitive compensation, equity, and benefits package.

About the Position

Primary Role and Responsibilities:

• Design and execute high-throughput protein engineering screens in collaboration with computational modeling group
• Develop methods to support protein evolution and affinity maturation
• Biophysical and functional characterization of protein biotherapeutics
• Implementation of automation to streamline laboratory workflows
• Maintain detailed records and contribute to intellectual property and regulatory filings
• Communicate and interact closely within cross-functional organization to understand needs and acheive short-term and long-term strategic goals

Qualifications:

• PhD degree in Biochemistry, Bioengineering, Molecular Biology or other related scientific discipline with 0-2 years related industry experience
• Advanced experience with one or various surface display technologies (yeast, phage, ribosome, mRNA…) for a high throughput protein engineering campaign as demonstrated by successful publication in a peer-reviewed journal
• Expertise in biochemical and biophysical techniques for characterizing protein-protein and protein-nucleic acid interactions (fluorescence polarization, SPR/BLI, AlphaLISA, HTRF, binding kinetics) and function (spectroscopy, enzyme kinetics, substrate-linked assays).
• Significant technical experience with various recombinant protein expression systems and subsequent purification of high-quality protein
• Experience in preparing NGS libraries and subsequent sequence analysis is preferred
• Previous work with protein evolution techniques is highly desired
• Proficiency in troubleshooting molecular biology (high throughput pooled and arrayed cloning) and protein expression workflows (expression optimization, affinity/ion exchange chromatography, FPLC)
• Attention to detail with rigorous scientific thinking and clear communication supported by appropriate documentation.
• Creative mindset to tackle difficult problems and propose solutions, as well as prioritize tasks across multiple projects
• Strong team player with a desire to contribute to innovative technologies and drive success in an early-stage company