Principal Scientist, Molecular Biology

At Johnson & Johnson, we believe health is everything. Our strength in healthcare innovation empowers us to build a world where complex diseases are prevented, treated, and cured, where treatments are smarter and less invasive, and solutions are personal. Through our expertise in Innovative Medicine and MedTech, we are uniquely positioned to innovate across the full spectrum of healthcare solutions today to deliver the breakthroughs of tomorrow, and profoundly impact health for humanity. Learn more at jnj.com.

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Job Function:

Discovery & Pre-Clinical/Clinical Development

Job Sub Function:

Biotherapeutics R&D

Job Category:

Scientific/Technology

All Job Posting Locations:

Spring House, Pennsylvania, United States of America

Job Description:

About Innovative Medicine:

Our expertise in Innovative Medicine is informed and inspired by patients, whose insights fuel our science-based advancements. Visionaries like you work on teams that save lives by developing the medicines of tomorrow.
 

Join us in developing treatments, finding cures, and pioneering the path from lab to life while championing patients every step of the way.
 

Learn more at https://www.jnj.com/innovative-medicine

We are searching for the best talent for a Principal Scientist, Molecular Biology to be onsite in Spring House, PA.
 

Purpose:
The in Vivo Antibody Discovery group within Biologics Discovery is responsible for lead antibody generation from immunized animals with state-of-the-art technologies including single B cell sorting, hybridoma, display and microfluidics. In this key role, the Principal Scientist will lead a team responsible for recovery of antibody sequences from selected hybridoma cells or single B cells. The candidate is expected to work with automation experts to automate NGS workflow for efficient sequence recovery. In-depth knowledge and extensive experience in molecular cloning of BCR using various approaches including RT-PCR, Topo cloning, in-fusion cloning, multiplex NGS, 10X genomics are required. A strong understanding of B-cell recovery methods such as Hybridoma technology and primary B-cell cloning will also be important. This is a critical function in a multifaceted Biologics Discovery organization committed to bringing the next generation of biotherapeutic molecules to patients. We are looking for a motivated candidate with scientific and technical expertise who can collaborate across discovery areas to bring value to our portfolio of innovative drug concepts. Proficiency to work within a highly coordinated, fast-paced environment will be a significant attribute. If this sounds like you, come join our team!

You will be responsible for:

• Provide day-to-day in-person guidance and mentoring within the V-Gene Recovery (VGR) team
• Lead antibody sequence recovery from selected hits of diverse sources, including hybridoma, single B cells, immune repertoires, and in vitro display platforms.
• Execute and optimize molecular biology workflows, including Next-Generation Sequencing (NGS) and conventional Sanger sequencing, to enable efficient and accurate antibody discovery.
• Work with automation experts to automate and streamline NGS workflows, identifying opportunities for process improvements to enhance sequencing accuracy and efficiency.
• Manage laboratory operations by maintaining equipment, ensuring sufficient inventory of supplies, and overseeing the routine maintenance of NGS instruments and related laboratory equipment.
• Conduct comprehensive data analysis, interpret sequencing results, and generate detailed reports. Bioinformatics experience is a plus.
• Present research findings in internal meetings, clearly communicating scientific results and providing insights into data interpretation.
• Collaborate with multidisciplinary teams across functional areas to advance therapeutic programs, contributing scientific expertise to multiple projects in a dynamic and fast-paced environment.
• Document work accurately and promptly using Elec