Research Assistant II - Laboratory of Dr. Kuo -Fen Lee

Job Summary

As a Research Assistant II, this position will be supporting the laboratory of Dr. Lee where they will focus on research support related to The Research Assistant II position is responsible for assisting with research in genetics and diseases in marmoset models. Working as part of a multidisciplinary team, she/he will assist in the transgenesis of marmosets and the analysis of the phenotype of the marmoset model. This includes neuroimaging, behavioral tests, stereotaxic injection, tissue and cell culturing, tissue collection and sectioning, immunohistochemistry, PCR, DNA and RNA extractions and genotyping. We are looking for a motivated individual willing to learn colony maintenance such as pedigree recording, pairing/breeding, and stress management. The candidate will also be trained in techniques required in assisting transgenesis of marmosets, including reproductive hormone profiling, oocyte collection, in vitro fertilization and embryo transfer. Other responsibilities include data analysis and maintenance of tissue, DNA, and other experimental samples. Individuals will be expected to determine with moderate direction the timing of experiments, analysis, and presentation of results.

Who We Are

The Salk Institute is an internationally renowned research institution that values diversity, equity, and inclusion. We seek bold and interactive leaders passionate about exploring new frontiers in science. Our collaborative community embraces diverse perspectives and unique life experiences, fostering innovation, and a sense of belonging. Together, we strive to improve the wellbeing of humanity through groundbreaking research.

Founded by Jonas Salk, developer of the first safe and effective polio vaccine, the Institute is an independent, nonprofit research organization and architectural landmark: small by choice, intimate by nature, and fearless in the face of any challenge. Salk's vibrant community comprises a diverse and talented group of individuals, each playing a crucial role in driving our mission forward. From visionary leaders to dedicated administrators and brilliant faculty members, the Institute is united by a shared passion for scientific exploration and innovation.

What Your Key Responsibilities Will Be

Transgenesis and Experimental Support for Marmoset Project

• Significant focus on procedures in transgenesis of marmosets, including reproductive hormone profiling, monitoring animal health during surgery, embryo and oocyte collection, sperm collection, in vitro fertilization of oocytes, embryo transfer, and care of neonate marmosets.
• With moderate direction from senior staff, responsible for handling marmosets to address routine to moderately complex concerns; evaluates experimental and procedural criteria; makes recommendations for changes; and employs techniques for obtaining results.
• Husbandry and maintenance of marmoset colonies and pedigree record keeping.
• May learn and perform pedigree analysis and establish breeding based on analysis to maintain genetic diversity.
• Performs animal monitoring under supervision of veterinary staff during surgery including but not limited to surgical preparation, anesthetic induction, animal monitoring, surgical assistance and post-operative recovery
• Assists with MRI/PET/CT imaging, stereotaxic injections of viruses and other reagents, behavioral tests, peripheral injections, biopsies, perfusions, and necropsies
• Perform cell and tissue collection and culture
• Assists with marmoset requests and shipments, maintains animal inventory and attends staff meetings related to scope of work
• Prepares and presents experimental results at lab meetings
• Other tasks and experiments as needed with expanding or changing scope of grants.

Lab Maintenance 

• Assist technicians and post-docs with experiments as needed
• Orders, stocks and organize incoming lab supplies as needed
• Attends staff meetings and assists with general problem solving
• Assists in writing of laboratory protocols for experiments
• Assists in Hazardous, Biohazardous, and Radioisotope waste disposal
• Assists in maintaining laboratory safety records
• Coordinates with vendors for problems regarding orders

What we Require

• Minimum 2 years experience, or equivalent combination of education and experience