RESEARCH TECHNICIAN II
Duke UniversityAbout the role
School of Medicine
Established in 1930, Duke University School of Medicine is the youngest of the nation's top medical schools. Ranked sixth among medical schools in the nation, the School takes pride in being an inclusive community of outstanding learners, investigators, clinicians, and staff where interdisciplinary collaboration is embraced and great ideas accelerate translation of fundamental scientific discoveries to improve human health locally and around the globe. Composed of more than 2,600 faculty physicians and researchers, nearly 2,000 students, and more than 6,200 staff, the Duke University School of Medicine along with the Duke University School of Nursing, and Duke University Health System comprise Duke Health, a world-class academic medical center. The Health System encompasses Duke University Hospital, Duke Regional Hospital, Duke Raleigh Hospital, Duke Health Integrated Practice, Duke Primary Care, Duke Home Care and Hospice, Duke Health and Wellness, and multiple affiliations.
Occupational Summary
Perform a variety of complex technical duties involved in conducting laboratory tests, experiments and determinations in support of Duke University School of medicine research operations.
The Aleks Tata lab in the Department of Surgery, Division of Surgical Sciences is seeking a Research Technician II to work on project focusing on cellular circuits in lung regeneration and disease.
Work Performed
Perform a variety of complex technical duties involved in conducting physical, chemical, biological and other research laboratory tests, experiments and determinations to obtain data for research purposes; compile, analyze, document and draw tentative conclusions from experimental results.
Confer with principal investigator to review work assignments and develop plans for research experiments; make minor modifications to existing laboratory procedures and techniques to meet the needs of the equipment as required. Review laboratory methods manual, scientific journals, abstracts and other literature for information applicable to research experiments.
Responsibilities include:
v Mice maintenance, breeding, genotyping
v Performs standard molecular biology techniques (cloning, PCR, DNA, RNA, and protein extraction, etc.)
v Packaging viruses (AAV, Lenti)
v Perform histological techniques (tissue processing, sectioning, immunofluorescence)
v Assist with performing small and short-term basic experiments and completing individual parts of an overall large experiment/project.
v Documents, compiles, and analyzes experimental results
v Prepare culture media, chemicals, reagents and solutions.
v Maintain sufficient inventory of material, supplies and equipment for performance of duties; clean and maintain laboratory equipment.
v Assist in training a new personnel and rotation students.
v Collect tissue samples from collaborators and process them for histology, cell cultures, including organoid cultures, and make frozen stocks for future use.
The above statements describe the general nature and level of work being performed by individuals assigned to this classification. This is not intended to be an exhaustive list of all responsibilities and duties required of personnel so classified.
Required Qualifications
Education/Training: Work generally requires a minimum of bachelor's degree in botany, biology, zoology, chemistry, molecular biology, biochemistry, genetics, or other directly related biomedical sciences. A master’s degree is not a requirement but that would be advantageous.
Experience: One to two years of laboratory training. OR AN EQUIVALENT COMBINATION OF RELEVANT EDUCATION AND/OR EXPERIENCE
The intent of this job description is to provide a representative and level of the types of duties and responsibilities that will be required of positions given this title and shall not be construed as a declaration of the total of the specific duties and responsibilities of any position. Employees may be directed to perform job-related tasks other than those specifically presented in this description.
Minimum Qualifications
Duke University is an Affirmative Action/Equal Opportunity Employer committed to providing employment opportunities without regard to an individual's age, color, disability, gender, gender expression, gender identity, genetic info
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