Research Scientist, Proteomics

Responsibilities and Duties

Corporate

• Follow SOPs and other applicable laboratory or company procedures

• Maintain confidential information consistent with Inotiv corporate policy

• Demonstrate Inotiv Core Values

General

• Interact with clients, other employees, and the community in a professional manner

• Support and participate in company initiatives

• Perform other duties as assigned

Specific

• Following the safety rules in the lab and maintaining a safe working environment.

• Operating lab equipment and performing analyses according to the Operation Manuals, SOPs, Best Practices, and training.

• Performing proteomic sample preparation in support of discovery and translational studies, including:

  • Protein extraction and lysis, reduction and alkylation, and enzymatic (e.g., tryptic) digestion.

  • Peptide cleanup and desalting (S-Trap, SP3, SPE) and protein/peptide quantification.

  • Preparing buffers, reagents, and stock solutions, and verifying their composition.

  • Preparing samples through LC-MS injection and assisting with data acquisition as instructed.

• Operating, programming, and maintaining automated liquid-handling and bead-based sample-preparation systems (e.g., KingFisher, Bravo) to support reproducible, plate-based, high-throughput proteomic processing.

• Recording results accurately in the ELN and contributing to data summaries and study documentation.

• Notifying the Lab Manager and/or Principal Investigator of any deviations from the study protocol/SOPs/Best Practices or unexpected processes preventing the proper completion of sample processing and documenting as required.

• Observing deadlines, producing timely results or communicating to management when deadlines cannot be accomplished.

• Performing assigned general lab tasks, including data logging, routine maintenance of equipment, and general housekeeping.

• Ordering reagents, parts and/or materials needed for the functioning of the Lab.  Handling, inventorying and managing incoming materials and samples, waste and shipping of samples to outside parties when needed.

• Ensuring training files are properly maintained by keeping his/her CV current and for providing copies of certificates or documentation of training.

• Demonstrating an understanding of Best Practices and SOPs, and applying Good Laboratory Practice principles (21 CFR Part 58) where studies are conducted under regulatory requirements.

Position Requirements

Education and experience

• BS, or MS, or equivalent degree in Chemistry, Biochemistry, Biology, Molecular Biology, or related field with qualifying laboratory experience in degree program

Skills and abilities

• A basic understanding of processes related to Bioanalysis including sample preparation, instrument / equipment operation and data processing and reporting.

• Experience with analysis of proteins and peptides by mass spectrometry, including protein-specific extraction, separation, and purification methods (e.g., in-solution or on-bead digestion, S-Trap, SP3, and C18/SPE peptide desalting).

• Experience with, or aptitude to learn, automated sample-preparation platforms, such as the Thermo KingFisher (magnetic bead–based protein/peptide cleanup and SP3) and the Agilent Bravo / AssayMAP (automated liquid handling and cartridge-based peptide desalting).

• Comfort working with plate-based, high-throughput workflows (e.g., 96-well processing) and large sample cohorts.

• An attitude for quality, an eye for detail, the ability to follow written instructions and work effectively with management to complete assigned tasks.

• Documentation discipline and the ability to follow SOPs and written protocols precisely; familiarity with GLP/GMP/GCP principles is a plus.

• Ability to use a computer and an electronic lab notebook (ELN) to record experimental detail, and to compile and maintain databases for records and inventory, utilizing the appropriate software.

• A working knowledge and awareness of general laboratory procedures.

• Good written and oral communication skills.

• Ability to adhere to all safety regulations and procedures.

Desired:

• Experience with software for analysis of mass spectrometry data.

Performance Requirements

General

• Ability to read, write, speak and understand English

Physical Activity

• Moderate p