Research Technician II/III

Company Description

UHN is Canada’s #1 hospital and the world’s #1 publicly funded hospital. With 10 sites and more than 44,000 TeamUHN members, UHN consists of Toronto General Hospital, Toronto Western Hospital, Princess Margaret Cancer Centre, Toronto Rehabilitation Institute, The Michener Institute of Education and West Park Healthcare Centre. As Canada's top research hospital, the scope of biomedical research and complexity of cases at UHN have made it a national and international source for discovery, education and patient care. UHN has the largest hospital-based research program in Canada, with major research in neurosciences, cardiology, transplantation, oncology, surgical innovation, infectious diseases, genomic medicine and rehabilitation medicine. UHN is a research hospital affiliated with the University of Toronto.

UHN’s vision is to build A Healthier World and it’s only because of the talented and dedicated people who work here that we are continually bringing that vision closer to reality.

www.uhn.ca

Job Description

Union: Non-Union
Number of Vacancies: 1
New or Replacement Position: New
Site: Princess Margaret Cancer Centre
Department: Research
Reports to: Principal Investigator
Salary Range: $31.03 - $46.84 hourly
Hours: 37.5 hours a week
Shifts: Monday - Friday
Status: Temporary Full-Time (1 year contract)
Closing Date: September 16, 2026 (repost)

Position Summary

Our lab is a multidisciplinary research program based at the Princess Margaret Cancer Centre, University Health Network, and closely integrated with the Structural Genomics Consortium. We operate at the intersection of clinical oncology, experimental biology, and functional genomics, with a focus on understanding chromatin architecture, epigenetic regulation, and tumor heterogeneity in cancer.

The lab uses 3D genome mapping, chromatin profiling, next-generation sequencing, patient-derived models, and multi-omic approaches to identify regulatory mechanisms that may inform precision oncology strategies, biomarker discovery, and epigenome-informed therapeutic approaches.

We are seeking a highly motivated Research Technician with strong wet-lab experience to support and execute experimental projects in cancer epigenomics and 3D genome biology. The successful candidate will contribute to projects involving chromatin conformation assays, functional epigenomics, next-generation sequencing library preparation, and patient-derived model systems.

This position is ideal for a candidate with hands-on experience in molecular biology, genomics, epigenomics, and cancer biology who is interested in working in a collaborative, translational research environment.

Duties

• Perform and optimize molecular biology and functional genomics experiments related to cancer epigenomics.
• Carry out chromatin and sequencing-based assays, including Hi-C, HiChIP, ATAC-seq, CUT&RUN, CUT&Tag, and related methods, as applicable.
• Prepare next-generation sequencing libraries across multiple assay types.
• Work with patient-derived cancer models, including organoids or related culture systems.
• Support sample processing, quality control, library preparation, and coordination of sequencing submissions.
• Maintain accurate experimental records, protocols, sample inventories, and laboratory documentation.
• Assist with troubleshooting and optimization of complex wet-lab protocols.
• Coordinate with computational scientists and other team members to support data generation, interpretation, and project progress.
• Contribute to the generation of figures, summaries, and experimental documentation for manuscripts, grants, presentations, and reports.
• Assist with ordering, reagent preparation, equipment maintenance, and general laboratory organization.
• Support training of junior lab members or students in established protocols, as appropriate.
• Contribute to a collaborative, rigorous, and well-organized lab environment.

Qualifications

• At minimum, Bachelor's degree in Molecular Biology, Cancer Biology, Genetics, Genomics, Biochemistry, Cell Biology, or a related field required 
  • Master's degree an asset
• Minimum of 3 years of relevant wet-lab research experience required 
• Strong hands-on experience with molecular biology and genomics techniques.
• Experience with next-generation sequencing library preparation.
• Experience with chromatin-based or epigenomics assays, such as ATAC-seq, CUT&RUN, CUT&Tag, Hi-C, HiChIP, Cap