Scheduled Hours

40

Position Summary

The Senior Scientist in the Cremins Lab will be responsible for both oversight and implementation of specific research projects as well as participating in collaborative projects with other lab members and developing experimental protocols and experimental cell and molecular biology standards for the laboratory. The scientist will work on projects broadly encompassing genome engineering in the form of degrons, dTAG systems, Cas9, Cas13, dCas, CRISPRoff, CRISPRon, and other new molecular technologies. The scientist will also lead experiments differentiating human iPSCs to neurons or organoids.

The candidate will be versatile with both genome engineering and cell biology skills for new tech dev projects, standardizing genome engineering pipelines, providing feedback to trainees on construct building, and developing publication quality workflows for the in situ sequencing data analysis. Candidate will work as a sub Team leader over the progression of genome engineering projects and be responsible for maintaining and organizing lab records, training junior scientists on technical assays, working with the PI to prepare and review manuscripts, and help with writing content for grant applications.

Collaborate with the team to design, optimize, and standardize experimental and analysis pipelines to accelerate high-quality publications. Review trainee data, provide feedback, and help ensure experiments are conducted in a single variable manner with positive and negative controls. The ideal candidate should have a strong background in: culturing induced pluripotent stem cells (iPSCs); differentiation of pluripotent cells into neuronal lineages; genome engineering using CRISPR/Cas9; molecular biology and biochemistry laboratory techniques (e.g., molecular cloning); cell biology approaches (e.g., immunofluorescence, RNA/DNA fluorescence in situ hybridization); routine chromatin techniques and sequencing-based technologies (e.g., RNA-seq, ATAC-seq, CUT&Tag/CUT&RUN; Hi-C). Prior experience in mentoring and supervising students and trainees is highly desirable. Prior experience in grant writing is advantageous.

This role is ideal for you if you enjoy the excitement of a bleeding-edge scientific laboratory and welcome the challenge of serving as a steady consistent imaging and computational scientist role in a fast-paced environment. Those who will thrive in the role are dedicated to collaboration and keen on playing an active role in maintaining excellent protocols, a clean, organized, and safe laboratory, and eager to lead themselves and others toward maintaining a warm, positive, and inclusive lab climate. Organized individuals with a can-do attitude, a passion for building unity and community within a larger laboratory, a knack for independent problem-solving and getting things done, and the ability to independently and positively create molecular and cellular data with attention to detail would excel in this role.

Job Description

Primary Duties & Responsibilities:

• Collaborates on designing, conducting and reporting of research projects.
• Assists with grant preparation and reporting. Guides lab members to write and edit methods sections and figures for research papers where the analysis is used in the manuscript .
• Prepares and submits papers on research. Leads multiple papers and major research papers at the center of the laboratories larger goals.
• Collaborates fluidly with computational experts to conduct efficient analyses leading to discovery in mammalian neuron genome structure-function data. In parallel, trains to achieve mastery and excellence in running code written by other members of the lab to analyze genomic and imaging data, for example calling DMLs in methylation data, calling compartments, TADs, subTADs, loops in Hi-C/Micro-C data, or peak calling of Cut&Run and Cut&Tag data.
• Responsible for deliverables of the lab genome engineering subTeam – including, but not limited to, conceptual selection of experimental strategies, protocol development, experimental design, validation, and training of other lab members in key bleeding edge experimental cell and molecular biology assays.
• Develops hypotheses to be tested and ways of testing it. Oversees and writes established SOPs for new and existing computational algorithms – iPSC differentiation to neurons, organoids, and assembloids.
• Complies with established safety procedures and maintains required documentation on laboratory and specimen conditions.
• Manages research project with other institutions and investigators.
• Performs other duties as assigned.

Working Conditions: