Sr. Scientist I, Complex In Vitro Models

Company Description

About AbbVie

AbbVie's mission is to discover and deliver innovative medicines and solutions that solve serious health issues today and address the medical challenges of tomorrow. We strive to have a remarkable impact on people's lives across several key therapeutic areas including immunology, oncology and neuroscience - and products and services in our Allergan Aesthetics portfolio. For more information about AbbVie, please visit us at www.abbvie.com. Follow @abbvie on LinkedIn, Facebook, Instagram, X and YouTube.

Job Description

AbbVie is seeking an experienced scientist to join our Immunology team to develop, characterize, and qualify complex in vitro models (CIVMs). This lab-based individual will be responsible for duties that include but are not limited to: routine cell culture for cell lines and primary cells, creation and maintenance of complex in vitro models (e.g. organoid, organ-on-chip) for multiple different tissue types with a focus on modeling autoimmune diseases and performing quantitative endpoint assays. This is an ideal position for a candidate who wants to be at the forefront of 3D tissue modeling technologies and can thrive in a diverse research environment; strong organizational, multi-tasking, collaboration, and communication skills are critical.

Key Responsibilities:

• Routine culture and maintenance of immortalized, stem, and primary cell cultures, both in standard formats as well as organoids or organ-on-chip formats. Experience with these systems, and with a variety of cell types (e.g. endothelial cells, intestinal organoids, fibroblasts, immune cells, etc.) is highly desirable.
• Isolation of primary human cells, including the isolation of primary cells from tissue and whole blood.
• Designing and conducting experiments to characterize in vitro models, including assessments of in vitro potency, cytotoxicity, transcript/protein abundance using techniques such as viability assays, microscopy, histopathology, and transcriptomics/proteomics. 
• Conducting experiments with a high level of independence and initiative and performing tasks with minimal direct supervision for routine methods.
• Independently schedule and prioritize workload to manage several tasks simultaneously and identify / utilize resources outside of your own group.
• Designing experiments to characterize models, generating foundational datasets to guide model selection for specific drug program needs.
• Maintaining detailed electronic documentation to ensure adherence to departmental procedures and contribute to final reports as needed.

Qualifications

• BS, MS, or PhD degree in cell or molecular biology, bioengineering, or a related field, with typically 10+ years (BS), 8+ years (MS), or 0+ years (PhD) of experience.
• Experience with standard cell and molecular biological assays such as ELISA/Luminex, nucleic acid extraction, qPCR, immunofluorescence staining and confocal microscopy, cell proliferation/viability assays, cytotoxicity assays, flow cytometry / FACS.
• Experience with primary-derived cell culture differentiation methodologies; phenotypic and functional characterization of tissue-specific and/or immunological cells; understanding of key biomarkers for core tissue cell populations, and/or tissue-resident or blood-derived immune cell populations.
• Excellent organizational and analytical skills, with the ability to independently troubleshoot, optimize, and innovate experimental approaches.
• Excellent communication skills, with an ability to work collaboratively in a matrixed research environment.

Preferred Qualifications:

• Experience working with human tissue samples and with isolating and characterizing cells such as epithelial, endothelial, and/or immunological (B cells, T cells, monocytes).
• Familiarity with automation, or high-throughput screening if relevant, as these are becoming increasingly important in advanced in vitro modeling.

Additional Information

Applicable only to applicants applying to a position in any location with pay disclosure requirements under state or local law:

• The compensation range described below is the range of possible base pay compensation that the Company believes in good faith it will pay for this role at the time of this posting based on the job grade for this position. Individual comp