Scientist I, Molecular Pathology and Imaging *PC 1595

Your Role:

As a member of the Research and Development team, you will have the opportunity to play a key, hands-on role within the functional area of ultra-high content imaging using the novel CliniMACS MACSima™, a fully automated instrument based on multiplex fluorescence microscopy. You will be responsible for tissue preparation, developing, optimizing and validating tissue staining procedures using immunofluorescence (IF), immunohistochemistry (IHC), and in-situ hybridization (ISH) methods. Furthermore, you will perform high-content image acquisition and analysis of tissues derived from human and animal tumors using MACS® iQView platform for multi-parameter analysis of imaging data at the resolution of a single cell. This work will provide mechanistic insights into discoveries in the field of cancer immunotherapy. The incumbent will have the opportunity to interact with a highly diverse and collaborative team of scientists focused on the research and development of novel cell and gene therapies, to contribute to scientific publications and development of commercial cell therapy products, and to grow professionally.

Essential Duties and Responsibilities:

• Responsible for animal and human tissue processing and staining for research and development projects. Work collaboratively with team members to achieve high quality of assay performance.
• Embed, section and stain fresh-frozen and fixed tissue specimens.
• Run optimization studies for staining reagents/methods.
• Perform low-content and high-content imaging of cells/tissues by optical and fluorescence microscopy.
• Independently perform image processing, interpretation, and laboratory data management.
• Work independently and in a team environment.
• Other duties as assigned

Requirements:

• Ph.D. degree in pathology, cancer biology, immunology, imaging, or a related field; A minimum of two years of relevant experience
• Skills in molecular pathology, including tissue processing, staining, and microscopy.
• Expertise in fresh and fixed tissue specimen preparation, including fresh tissue fixation, paraffin embedding, sectioning, mounting slides, and pre-staining slide processing.
• Experience in H&E and other standard histopathology staining, IHC, ISH and IF on fresh frozen and formalin-fixed paraffin-embedded tissue.
• Experience in image analysis using commercial or open-source image processing suites.
• Experience in isolation and culture of primary cells, multicolor flow cytometry and cell-based assays is a plus.
• Experience in high-content data analysis, machine learning (large language models), and automation of data workflows is also a plus, with an aim to understand the tumor microenvironment is also desirable.

Physical Demands:

The physical demands described here are representative of those that must be met by an employee to successfully perform the essential functions of this job. Reasonable accommodations may be made to enable individuals with disabilities to perform the essential functions. While performing the duties of this job, the employee is regularly required to walk; use hands to finger, handle, or feel; reach with hands and arms, talk, and hear. The employee is required to stand; climb or balance and stoop, kneel, crouch. The employee must occasionally lift and/or move up to 50 pounds. Specific vision abilities required by this job include close vision, color vision, peripheral vision, depth perception and ability to adjust focus. Must have ability to handle more than one task at a time and must work at a rapid pace while maintaining attention to detail.

Work Environment:

The work environment characteristics described here are representative of those an employee encounters while performing the essential functions of this job. This job is performed in a temperature-controlled facility without exposure to extreme hot or cold temperatures. Generally, this job is performed in a sanitized laboratory setting and routinely uses standard medical and research equipment such as centrifuges, biosafety cabinets, microscopes, incubators, pipettes and computers. While performing the duties of this job, the employee is frequently exposed to blood, viruses, bacteria, body fluids and other tissues. Employees entering and leaving the laboratory must wear appropriate clothing and protective equipment such as scrubs, coveralls, masks, glasses and gloves. The noise level in the work environment is usually moderate.

The hiring range for this position is expected to fall between $107,800 -