Research Tech III - Medical Toxicology

SHIFT:

Any (United States of America)

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A Brief Overview
This role participates in planned research projects by performing research protocols with minimal direction and guidance from the principal investigator, program director or senior scientists. May exercise moderate discretion and judgment in the execution and interpretation of experiments, and of data derived.

We have an exciting opportunity for a highly motivated small animal/biomolecular technician to join the Resuscitation Science Center (RSC) at The Children’s Hospital of Philadelphia, Philadelphia, PA, as a member and participant in our molecular lab research, with a specific focus on small animal (rat) work and biomolecular methods at the bench. Our research focuses on advanced therapies for treating cardiac arrest and traumatic brain injuries (TBI) along with potential therapeutics for mitochondrial diseases to reduce neurological and mitochondrial damage. Clinically, our research helps to improve pediatric survival outcomes and reduce neurological deficits post-cardiac arrest/TBI, in addition to growing the scope of knowledge related to mitochondrial disorder therapies. Our focus is on critical illness: Traumatic Brain Injury, Cardiac Arrest, Extracorporeal Life Support, Cardiopulmonary Bypass, and Toxicology. Our laboratory is truly translational laboratory that span multiple models of critical illness that range from cell culture models to small animal and large animals (swine).

 

For this particular position, the focus is related to animal models of poisoning under Dr. Jang who is a medical toxicologist and research collaborator with Dr. Kilbaugh (RSC Director). His research is focused in the area of carbon monoxide poisoning and organophosphate using swine and rodent models. The primary duties of this position will focus on rodent work involving surgical procedures, behavioral assessment and support related to this work such as maintaining small animals supplies. Secondary responsibilities will include some biomolecular experiments mainly involved mitochondrial assays.

 

The successful candidate should possess exceptional organization, communication, time management, and critical thinking skills and be willing to work in both a team and independent setting. Prior lab experience in cell/molecular or other biological studies is required. A willingness to and/or experience with working hands on with small animals for experiments is preferred but not required. Due to the time sensitive nature of the experiments, the successful candidate must be willing to work flexible hours as dictated by the varying timelines of the different studies/experiments.

Job Responsibilities:

• Small animal work (primarily rats) that involved perioperative procedures, instrumentation, neurobehavioral assessment. Experience in this area is highly desirable and preferred.
• Perform or assist with basic necropsy animal procedures (dissection, tissue collection, and sample collection) for further downstream analysis
• Perform mitochondrial specific assays such as high-resolution respirometry, microdialysis, citrate synthase activity on samples collected from animal experiments
• Help store and log biobanked tissue samples from studies in sample management organization software (Freezerworks)
• Compile and analyze data using Microsoft Office programs, and possibly other data programs including but not limited to Graphpad Prism and Matlab
• Design and execute experiments in a timely manner under the leadership of the Project Lead and/or PI.
• Applicant must possess strong communicative skills; prioritize initiatives and projects across all stages while team building through excellent problem-solving skills.
• Ability to objectively critique results and present as valid or questionable, taking into account all aspects of study design, technical issues, and limitations of wo